Advancing high-throughput gene synthesis technology
Identifieur interne : 002845 ( Main/Exploration ); précédent : 002844; suivant : 002846Advancing high-throughput gene synthesis technology
Auteurs : Jingdong Tian [États-Unis] ; Kuosheng Ma [États-Unis] ; Ishtiaq Saaem [États-Unis]Source :
- Molecular BioSystems [ 1742-206X ] ; 2009.
English descriptors
- Teeft :
- Adjacent cathodes, Array synthesis, Assembly reaction, Biomedical, Biomedical engineering, Biomedical research, Biomedical research award, Biomolecular engineering, Biosyst, Biotechnol, Building blocks, Chemical synthesis, Chip surface, Chip synthesis gated, Conventional gene synthesis, Correct sequences, Current protocols, Deprotection, Deprotection step, Digital micromirror array, Digital photolithography, Duke university, Electrochemical array, Enzymatic methods, Error removal, Escherichia coli, Exible, Gene, Gene assembly, Gene circuits, Gene construction, Gene sequence, Gene synthesis, Gene synthesis technology, Genome, Genome sciences, Incorrect sequences, Inkjet, Inkjet head, Ishtiaq saaem, Large numbers, Length records, Ligase chain reaction, Light exposure, Light patterns, Metabolic engineering, Methods appl, Methods enzymol, Microarray, Microarray fabrication, Microarray synthesis, Microarrays, Microchip, Microvalve array, Mismatch, Mismatch binding proteins, Mismatch cleaving enzymes, Molecular biosystems, Monomer, Multiple genes, Nucleic, Nucleic acid chemistry, Nucleic acids, Nucleotide, Oligo sequences, Oligonucleotide, Oligonucleotide chain, Oligonucleotide sequences, Oligonucleotide synthesis, Oligonucleotides, Oligos, Pdms, Peroxy anion, Phosphoramidite, Phosphoramidite method, Phosphoramidite monomer, Phosphoramidite monomers, Photolithography, Physical masks, Piezoelectric inkjet head, Polymerase chain reaction, Primer, Primer pair, Protection groups, Reagent, Royal society, Size exclusion, Small fraction, Solid support, Solution photogenerated acids, Synthesis, Synthesis cycle, Synthesis procedure, Synthesizer, Synthetic biology, Synthetic genes, Synthetic oligonucleotides, Texas instruments, Throughput, Total synthesis.
Abstract
The emerging field of synthetic biology is generating insatiable demands for synthetic genes, which far exceed existing gene synthesis capabilities. This review discusses the current methods of chemical DNA synthesis and gene assembly, as well as the latest engineering tools, technologies and trends which could potentially lead to breakthroughs in the development of accurate, low-cost and high-throughput gene synthesis technology. The capability of generating unlimited supplies of DNA molecules of any sequence or size will transform biomedical research in the near future.
Url:
DOI: 10.1039/b822268c
Affiliations:
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Le document en format XML
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<term>Biomedical research</term>
<term>Biomedical research award</term>
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<term>Error removal</term>
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<term>Exible</term>
<term>Gene</term>
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<term>Oligonucleotide sequences</term>
<term>Oligonucleotide synthesis</term>
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<front><div type="abstract">The emerging field of synthetic biology is generating insatiable demands for synthetic genes, which far exceed existing gene synthesis capabilities. This review discusses the current methods of chemical DNA synthesis and gene assembly, as well as the latest engineering tools, technologies and trends which could potentially lead to breakthroughs in the development of accurate, low-cost and high-throughput gene synthesis technology. The capability of generating unlimited supplies of DNA molecules of any sequence or size will transform biomedical research in the near future.</div>
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